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In vivo biodistribution of PHA‐EVs in inflammatory animal models with tumour and RA. (a) In vivo fluorescence images of <t>Cy5.5‐EVs</t> and Cy5.5‐PHA‐EVs in CIA mice. (b) Fluorescence intensity of Cy5.5‐EVs and Cy5.5‐PHA‐EVs in the inflamed joints of CIA mice as a function of time ( n = 3). (c) Distribution of CD44 and EV in the inflamed synovium of CIA mice. (d) Whole‐body fluorescence images of Cy5.5‐EVs and Cy5.5‐PHA‐EVs in PC3 tumour‐bearing mice. (e) Fluorescence intensity of Cy5.5‐EVs and Cy5.5‐PHA‐EVs in the tumour site as a function of time ( n = 3). (f) Distribution of CD44 and EV in tumour tissue. (b and e) * P < 0.05, analyzed by two‐way ANOVA. Error bars represent standard error
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In vivo biodistribution of PHA‐EVs in inflammatory animal models with tumour and RA. (a) In vivo fluorescence images of <t>Cy5.5‐EVs</t> and Cy5.5‐PHA‐EVs in CIA mice. (b) Fluorescence intensity of Cy5.5‐EVs and Cy5.5‐PHA‐EVs in the inflamed joints of CIA mice as a function of time ( n = 3). (c) Distribution of CD44 and EV in the inflamed synovium of CIA mice. (d) Whole‐body fluorescence images of Cy5.5‐EVs and Cy5.5‐PHA‐EVs in PC3 tumour‐bearing mice. (e) Fluorescence intensity of Cy5.5‐EVs and Cy5.5‐PHA‐EVs in the tumour site as a function of time ( n = 3). (f) Distribution of CD44 and EV in tumour tissue. (b and e) * P < 0.05, analyzed by two‐way ANOVA. Error bars represent standard error
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Fig. 5. Representative confocal microscopy images of A549 lung epithelial cells incubated with biotin- PEG2k-b-GA30/10 – Cy-5-lysozyme complexes for 1 h, followed by a subsequent application of Alexa 488- conjugated streptavidin (green) in fixed and per meabilized cells (a, b and c). Pearson’s Correlation Coefficient analysis was used for intracellular co- localization studies of labelled protein <t>(Cy5-lyso</t> zyme, red) and AlexaFluor 488 (green) conjugated streptavidin; nuclear stain (DAPI, blue). (For inter pretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Sulfo Cyanine 5 Cy5 Succinimidyl Nhs Ester, supplied by Lumiprobe, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 5. Representative confocal microscopy images of A549 lung epithelial cells incubated with biotin- PEG2k-b-GA30/10 – Cy-5-lysozyme complexes for 1 h, followed by a subsequent application of Alexa 488- conjugated streptavidin (green) in fixed and per meabilized cells (a, b and c). Pearson’s Correlation Coefficient analysis was used for intracellular co- localization studies of labelled protein <t>(Cy5-lyso</t> zyme, red) and AlexaFluor 488 (green) conjugated streptavidin; nuclear stain (DAPI, blue). (For inter pretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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Fig. 5. Representative confocal microscopy images of A549 lung epithelial cells incubated with biotin- PEG2k-b-GA30/10 – Cy-5-lysozyme complexes for 1 h, followed by a subsequent application of Alexa 488- conjugated streptavidin (green) in fixed and per meabilized cells (a, b and c). Pearson’s Correlation Coefficient analysis was used for intracellular co- localization studies of labelled protein <t>(Cy5-lyso</t> zyme, red) and AlexaFluor 488 (green) conjugated streptavidin; nuclear stain (DAPI, blue). (For inter pretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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Fig. 5. Representative confocal microscopy images of A549 lung epithelial cells incubated with biotin- PEG2k-b-GA30/10 – Cy-5-lysozyme complexes for 1 h, followed by a subsequent application of Alexa 488- conjugated streptavidin (green) in fixed and per meabilized cells (a, b and c). Pearson’s Correlation Coefficient analysis was used for intracellular co- localization studies of labelled protein <t>(Cy5-lyso</t> zyme, red) and AlexaFluor 488 (green) conjugated streptavidin; nuclear stain (DAPI, blue). (For inter pretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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Fig. 5. Representative confocal microscopy images of A549 lung epithelial cells incubated with biotin- PEG2k-b-GA30/10 – Cy-5-lysozyme complexes for 1 h, followed by a subsequent application of Alexa 488- conjugated streptavidin (green) in fixed and per meabilized cells (a, b and c). Pearson’s Correlation Coefficient analysis was used for intracellular co- localization studies of labelled protein <t>(Cy5-lyso</t> zyme, red) and AlexaFluor 488 (green) conjugated streptavidin; nuclear stain (DAPI, blue). (For inter pretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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Fig. 5. Representative confocal microscopy images of A549 lung epithelial cells incubated with biotin- PEG2k-b-GA30/10 – Cy-5-lysozyme complexes for 1 h, followed by a subsequent application of Alexa 488- conjugated streptavidin (green) in fixed and per meabilized cells (a, b and c). Pearson’s Correlation Coefficient analysis was used for intracellular co- localization studies of labelled protein <t>(Cy5-lyso</t> zyme, red) and AlexaFluor 488 (green) conjugated streptavidin; nuclear stain (DAPI, blue). (For inter pretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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Fig. 5. Representative confocal microscopy images of A549 lung epithelial cells incubated with biotin- PEG2k-b-GA30/10 – Cy-5-lysozyme complexes for 1 h, followed by a subsequent application of Alexa 488- conjugated streptavidin (green) in fixed and per meabilized cells (a, b and c). Pearson’s Correlation Coefficient analysis was used for intracellular co- localization studies of labelled protein <t>(Cy5-lyso</t> zyme, red) and AlexaFluor 488 (green) conjugated streptavidin; nuclear stain (DAPI, blue). (For inter pretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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Fig. 5. Representative confocal microscopy images of A549 lung epithelial cells incubated with biotin- PEG2k-b-GA30/10 – Cy-5-lysozyme complexes for 1 h, followed by a subsequent application of Alexa 488- conjugated streptavidin (green) in fixed and per meabilized cells (a, b and c). Pearson’s Correlation Coefficient analysis was used for intracellular co- localization studies of labelled protein <t>(Cy5-lyso</t> zyme, red) and AlexaFluor 488 (green) conjugated streptavidin; nuclear stain (DAPI, blue). (For inter pretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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Image Search Results


Journal: STAR Protocols

Article Title: Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue

doi: 10.1016/j.xpro.2021.100971

Figure Lengend Snippet:

Article Snippet: Cy-3B Mono NHS-ester , Cytiva , PA63101.

Techniques: Recombinant, Saline, Electron Microscopy, Plasmid Preparation, Software, Imaging, Control, Mass Measurement, Capsules, Microscopy, Staining, Adhesive, Spectrophotometry

In vivo biodistribution of PHA‐EVs in inflammatory animal models with tumour and RA. (a) In vivo fluorescence images of Cy5.5‐EVs and Cy5.5‐PHA‐EVs in CIA mice. (b) Fluorescence intensity of Cy5.5‐EVs and Cy5.5‐PHA‐EVs in the inflamed joints of CIA mice as a function of time ( n = 3). (c) Distribution of CD44 and EV in the inflamed synovium of CIA mice. (d) Whole‐body fluorescence images of Cy5.5‐EVs and Cy5.5‐PHA‐EVs in PC3 tumour‐bearing mice. (e) Fluorescence intensity of Cy5.5‐EVs and Cy5.5‐PHA‐EVs in the tumour site as a function of time ( n = 3). (f) Distribution of CD44 and EV in tumour tissue. (b and e) * P < 0.05, analyzed by two‐way ANOVA. Error bars represent standard error

Journal: Journal of Extracellular Vesicles

Article Title: Bioorthogonally surface‐edited extracellular vesicles based on metabolic glycoengineering for CD44‐mediated targeting of inflammatory diseases

doi: 10.1002/jev2.12077

Figure Lengend Snippet: In vivo biodistribution of PHA‐EVs in inflammatory animal models with tumour and RA. (a) In vivo fluorescence images of Cy5.5‐EVs and Cy5.5‐PHA‐EVs in CIA mice. (b) Fluorescence intensity of Cy5.5‐EVs and Cy5.5‐PHA‐EVs in the inflamed joints of CIA mice as a function of time ( n = 3). (c) Distribution of CD44 and EV in the inflamed synovium of CIA mice. (d) Whole‐body fluorescence images of Cy5.5‐EVs and Cy5.5‐PHA‐EVs in PC3 tumour‐bearing mice. (e) Fluorescence intensity of Cy5.5‐EVs and Cy5.5‐PHA‐EVs in the tumour site as a function of time ( n = 3). (f) Distribution of CD44 and EV in tumour tissue. (b and e) * P < 0.05, analyzed by two‐way ANOVA. Error bars represent standard error

Article Snippet: Cy5.5 NHS ester dye was purchased from GE Healthcare (IL, USA).

Techniques: In Vivo, Fluorescence

Fig. 5. Representative confocal microscopy images of A549 lung epithelial cells incubated with biotin- PEG2k-b-GA30/10 – Cy-5-lysozyme complexes for 1 h, followed by a subsequent application of Alexa 488- conjugated streptavidin (green) in fixed and per meabilized cells (a, b and c). Pearson’s Correlation Coefficient analysis was used for intracellular co- localization studies of labelled protein (Cy5-lyso zyme, red) and AlexaFluor 488 (green) conjugated streptavidin; nuclear stain (DAPI, blue). (For inter pretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Journal: Journal of controlled release : official journal of the Controlled Release Society

Article Title: Biotin receptor-mediated intracellular delivery of synthetic polypeptide-protein complexes.

doi: 10.1016/j.jconrel.2023.03.051

Figure Lengend Snippet: Fig. 5. Representative confocal microscopy images of A549 lung epithelial cells incubated with biotin- PEG2k-b-GA30/10 – Cy-5-lysozyme complexes for 1 h, followed by a subsequent application of Alexa 488- conjugated streptavidin (green) in fixed and per meabilized cells (a, b and c). Pearson’s Correlation Coefficient analysis was used for intracellular co- localization studies of labelled protein (Cy5-lyso zyme, red) and AlexaFluor 488 (green) conjugated streptavidin; nuclear stain (DAPI, blue). (For inter pretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: To monitor cellular internalization of the protein cargo, lysozyme used in this H. Li et al. Journal of Controlled Release 357 (2023) 333–341 experiment was fluorescently labelled using sulfo-Cyanine 5 (Cy5) succinimidyl (NHS) ester (Lumiprobe), according to the manufacturer’s protocol (as described in Supporting information, Methods).

Techniques: Confocal Microscopy, Incubation, Staining